ABSTRACT
This research was aimed to investigate anti-hyperglycemic effects of two Lactobacillus spp. on alloxan induced diabetic rats. Alloxan was administered intraperitoneally to induce the diabetic conditions in experimental rats. Animals were treated with oral administration of Lactobacillus spp., such as L. plantarum and L. acidophilus at the dose of 108 CFU/ml. As a result, administration of Lactobacillus spp. significantly [P<0.05] lowered blood glucose levels in diabetic rats by [201-220mg/dl] as compared to diabetic control [265mg/dl]. Also, both the Lactobacillus spp. were able to reduce body weight of experimental animals as compared to control group, suggesting potent anti-hyperglycemic effect of Lactobacillus spp. in terms of their anti-diabetic potential
ABSTRACT
In this study, heat-treated cucumber juice was assessed for its protective effect on blood alcohol levels and hepatic alcohol metabolic enzyme system in experimental rats. Initially, during detoxification of alcohol, all groups were orally dosed to 22% alcohol [6ml/kg body weight] along with different concentrations of heat-treated cucumber juice [10, 100 and 500mg/kg] and commercial goods for hangover-removal on sale [2ml/kg]. Cucumber juice was dosed before 30 min, and simultaneously after SOmin of alcohol administration, and its hepatoprotective effect on blood alcohol levels and hepatic alcohol metabolic enzyme system in experimental rats was evaluated. As a result, after 7h, remarkable reduction was found in the blood alcohol levels for all concentrations of cucumber juice treatment. Treatment with cucumber juice resulted in increasing dehydrogenase [ADH] and acetaldehyde dehydrogenase [ALDH] enzymatic activities in rat liver at 9h after alcohol administration thereby stimulated blood alcohol metabolism as compared with control group. The effect of heat-treated cucumber juice on alcohol detoxification was observed only in the rats treated before SOmin from alcohol administration. These findings indicate that heat-treated cucumber juice has significant protective effect on alcohol detoxification in experimental rats, suggesting its usefulness in the treatment of liver injury caused by alcohol consumption
ABSTRACT
This research reports first time antiviral activity of sugiol, a diterpenoid isolated from Metasequoia glyptostroboides in terms of its ability to inhibit in vitro growth of HiNi influenza virus. Antiviral potential of sugiol was evaluated through cytopathogenic reduction assay using Madin-Darby canine kidney [MDCK] cell line. Sugiol [500 jug/ml] was found to exhibit considerable anti-cytopathic effect on MDCK cell line confirming its antiviral efficacy against HiNi influenza virus. These findings strongly reinforce the uf gestion that sugiol could be a candidate of choice in combinational regimen with potential antiviral efficacy
ABSTRACT
OBJECTIVE@#To examine the individual and synergistic anti-listerial effect of nisin and leaf essential oil of Metasequoia glyptostroboides (M. glyptostroboides) against one of the leading foodborne pathogens Listeria monocytogenes (L. monocytogenes) ATCC 19116 in milk samples.@*METHODS@#The whole (8%), low (1%) and skim (no fat content) milk samples were inoculated with L. monocytogenes ATCC 19116 along with leaf essential oil of M. glyptostroboides or nisin alone as well in combinations.@*RESULTS@#In this study, the leaf essential oil at the concentrations of 2% and 5% revealed strong anti-listerial effect against L. monocytogenes ATCC 19116 in all categories of milk samples. Nisin at the concentrations of 250 and 500 IU/mL displayed a strong inhibitory effect against ATCC 19116 as compared to the control group. Additionally, synergistic combinations of leaf essential oil (1%) and nisin (62.5, 125, 250 and 500 IU/mL) also had a remarkable anti-listerial synergism in all the tested milk samples including whole, low and skim milk after 14 days.@*CONCLUSIONS@#As a major finding, the leaf essential oil of M. glyptostroboides might be a useful candidate for using in food industry to control the growth of foodborne pathogenic bacteria as confirmed by its potent anti-listerial synergistic effect with nisin against L. monocytogenes ATCC 19116 in different milk samples.
Subject(s)
Animals , Anti-Bacterial Agents , Chemistry , Pharmacology , Colony Count, Microbial , Cupressaceae , Chemistry , Food Microbiology , Listeria monocytogenes , Milk , Microbiology , Nisin , Chemistry , Pharmacology , Oils, Volatile , Chemistry , Pharmacology , Plant Extracts , Chemistry , Pharmacology , Plant Leaves , ChemistryABSTRACT
OBJECTIVE@#To investigate the antioxidant efficacy of a biologically active diterpenoid compound sugiol isolated from Metasequoia glyptostroboides (M. glyptostroboides) in various antioxidant models.@*METHODS@#An abietane type diterpenoid sugiol, isolated from ethyl acetate extract of M. glyptostroboides cones, was analyzed for its antioxidant efficacy as reducing power ability and lipid peroxidation inhibition as well as its ability to scavenge free radicals such as 1,1-diphenyl-2-picryl hydrazyl, nitric oxide, superoxide and hydroxyl radicals.@*RESULTS@#The sugiol showed significant and concentration-dependent antioxidant and free radical scavenging activities. Consequently, the sugiol exerted lipid peroxidation inhibitory effect by 76.5% as compared to α-tocopherol (80.13%) and butylated hydroxyanisole (76.59%). In addition, the sugiol had significant scavenging activities of 1,1-diphenyl-2-picryl hydrazyl, nitric oxide, superoxide and hydroxyl free radicals in a concentration-dependent manner by 78.83%, 72.42%, 72.99% and 85.04%, when compared to the standard compound ascorbic acid (81.69%, 74.62%, 73.00% and 73.79%) and α-tocopherol/butylated hydroxyanisole (84.09%, 78.61%, 74.45% and 70.02%), respectively.@*CONCLUSIONS@#These findings justify the biological and traditional uses of M. glyptostroboides or its secondary metabolites as confirmed by its promising antioxidant efficacy.